分类： 天文学 >> 天文学 提交时间： 2024-03-29 合作期刊: 《Research in Astronomy and Astrophysics》
摘要： Cloud cover plays a pivotal role in assessing observational conditions for astronomical site-testing. Except for the fraction of observing time, its fragmentation also wields a significant influence on the quality of nighttime sky clarity. In this article, we introduce the function , designed to comprehensively capture both the fraction of available observing time and its continuity. Leveraging in situ measurement data gathered at the Muztagh-Ata site between 2017 and 2021, we showcase the effectiveness of our approach. The statistical result illustrates that the Muztagh-Ata site affords approximately 122 nights that were absolutely clear and 205 very good nights annually, corresponding to Γ ≥ 0.9 and Γ ≥ 0.36 respectively.
分类： 生物学 >> 生物物理学 提交时间： 2016-05-12
Yang, Mingkun
Wang, Yan
Chen, Ying
Mo, Ran
Ge, Feng
Cheng, Zhongyi
Gu, Jing
Deng, Jiaoyu
Wang, Xude
Bi, Lijun
Chen, Chuangbin
摘要： Mycobacterium tuberculosis (Mtb), the causative agent of human tuberculosis, remains one of the most prevalent human pathogens and a major cause of mortality worldwide. Metabolic network is a central mediator and defining feature of the pathogenicity of Mtb. Increasing evidence suggests that lysine succinylation dynamically regulates enzymes in carbon metabolism in both bacteria and human cells; however, its extent and function in Mtb remain unexplored. Here, we performed a global succinylome analysis of the virulent Mtb strain H37Rv by using high accuracy nano-LC-MS/MS in combination with the enrichment of succinylated peptides from digested cell lysates and subsequent peptide identification. In total, 1545 lysine succinylation sites on 626 proteins were identified in this pathogen. The identified succinylated proteins are involved in various biological processes and a large proportion of the succinylation sites are present on proteins in the central metabolism pathway. Site-specific mutations showed that succinylation is a negative regulatory modification on the enzymatic activity of acetylCoA synthetase. Molecular dynamics simulations demonstrated that succinylation affects the conformational stability of acetyl-CoA synthetase, which is critical for its enzymatic activity. Further functional studies showed that CobB, a sirtuin-like deacetylase in Mtb, functions as a desuccinylase of acetyl-CoA synthetase in in vitro assays. Together, our findings reveal widespread roles for lysine succinylation in regulating metabolism and diverse processes in Mtb. Our data provide a rich resource for functional analyses of lysine succinylation and facilitate the dissection of metabolic networks in this life-threatening pathogen.
点击量
通过
点击量
下载量
评论
待评议
